rat ip Search Results


anti cd59 antibody  (Miltenyi Biotec)
90
Miltenyi Biotec anti cd59 antibody
Anti Cd59 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd59  (Sino Biological)
93
Sino Biological cd59
Cd59, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat ip  (Elabscience Biotechnology)
94
Elabscience Biotechnology rat ip
Rat Ip, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcl10 protein  (R&D Systems)
93
R&D Systems cxcl10 protein
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Cxcl10 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcl10 ip 10 elisa kits  (R&D Systems)
90
R&D Systems cxcl10 ip 10 elisa kits
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Cxcl10 Ip 10 Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd59a  (Miltenyi Biotec)
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Miltenyi Biotec cd59a
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Cd59a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids encoding rat ip 3-kinase b and the invariant chain of the mhc class ii complex  (Promega)
90
Promega plasmids encoding rat ip 3-kinase b and the invariant chain of the mhc class ii complex
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Plasmids Encoding Rat Ip 3 Kinase B And The Invariant Chain Of The Mhc Class Ii Complex, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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purified igg fraction of rabbit anti rat interferon-inducible protein - ip-10  (Torrey Pines Biolabs)
90
Torrey Pines Biolabs purified igg fraction of rabbit anti rat interferon-inducible protein - ip-10
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Purified Igg Fraction Of Rabbit Anti Rat Interferon Inducible Protein Ip 10, supplied by Torrey Pines Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant cotton rat cxcl10/ip-10 protein, cf  (Bio-Techne corporation)
90
Bio-Techne corporation recombinant cotton rat cxcl10/ip-10 protein, cf
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Recombinant Cotton Rat Cxcl10/Ip 10 Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody  (R&D Systems)
90
R&D Systems monoclonal antibody
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcl10  (R&D Systems)
94
R&D Systems cxcl10
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Cxcl10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant rat cd59 protein  (Sino Biological)
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Sino Biological recombinant rat cd59 protein
FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, <t>Cxcl10,</t> Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.
Recombinant Rat Cd59 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, Cxcl10, Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Phenotypic polarization of activated astrocytes: the critical role of lipocalin-2 in the classical inflammatory activation of astrocytes.

doi: 10.4049/jimmunol.1301637

Figure Lengend Snippet: FIGURE 1. Identification of phenotypic polarization in primary astrocyte cultures based on gene-expression profiles. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN-g (50 U/ml), IL-4 (10 ng/ml), ICs (OVA [75 mg/ml] plus anti- OVA Ab [150 mg/ml]) plus LPS (100 ng/ ml), or IL-10 (10 ng/ml) for 8 h, and total RNAs were isolated. (A) The mRNA levels of phenotypic markers (Il-1b, Inos, Tnf-a, Cxcl10, Il-12, Il-23, Il-10, Arg1, Mrc1, Il- 1ra, Fizz1, and Ym1) were determined by real-time RT-PCR. (B) Polarized classical versus alternative activation was also as- sessed by the ratio of expressed mRNA markers (Il-1b/Il-1ra, Inos/Arg1, Tnf-a /Il- 10). Gapdh was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus untreated control.

Article Snippet: Recombinant mouse TNF-a and CXCL10 protein (R&D Systems) were used as standards.

Techniques: Gene Expression, Isolation, Quantitative RT-PCR, Activation Assay, Control

FIGURE 2. Assessment of NO pro- duction, TNF-a/CXCL10 secretion, ex- pression of ARG1/MRC1 proteins, and GFAP levels following exposure to ei- ther classical or alternative activation stimuli. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN- g (50 U/ml), IL-4 (10 ng/ml), IC (OVA [75 mg/ml] plus OVA Ab [150 mg/ml]) plus LPS (100 ng/ml), or IL-10 (10 ng/ ml) for 24 h. (A) Concentrations of ni- trite, TNF-a protein, and CXCL10 pro- tein in culture media were measured using Griess reagent or specific ELISA. (B) ARG1 and MRC1 protein expres- sion was detected by flow cytometry using Abs specific for ARG1 or MRC1. Mean fluorescence intensity (MFI) val- ues are also shown. Negative control is the measurement without specific Ab or with a control Ab. GFAP mRNA or protein levels were evaluated by RT- PCR (C) or Western blotting (D), re- spectively. Gapdh or a-tubulin was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus un- treated control.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Phenotypic polarization of activated astrocytes: the critical role of lipocalin-2 in the classical inflammatory activation of astrocytes.

doi: 10.4049/jimmunol.1301637

Figure Lengend Snippet: FIGURE 2. Assessment of NO pro- duction, TNF-a/CXCL10 secretion, ex- pression of ARG1/MRC1 proteins, and GFAP levels following exposure to ei- ther classical or alternative activation stimuli. Primary astrocyte cultures were treated with LPS (100 ng/ml) plus IFN- g (50 U/ml), IL-4 (10 ng/ml), IC (OVA [75 mg/ml] plus OVA Ab [150 mg/ml]) plus LPS (100 ng/ml), or IL-10 (10 ng/ ml) for 24 h. (A) Concentrations of ni- trite, TNF-a protein, and CXCL10 pro- tein in culture media were measured using Griess reagent or specific ELISA. (B) ARG1 and MRC1 protein expres- sion was detected by flow cytometry using Abs specific for ARG1 or MRC1. Mean fluorescence intensity (MFI) val- ues are also shown. Negative control is the measurement without specific Ab or with a control Ab. GFAP mRNA or protein levels were evaluated by RT- PCR (C) or Western blotting (D), re- spectively. Gapdh or a-tubulin was used as an internal control. Results are mean 6 SD (n = 3). *p , 0.05, versus un- treated control.

Article Snippet: Recombinant mouse TNF-a and CXCL10 protein (R&D Systems) were used as standards.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Cytometry, Negative Control, Control, Reverse Transcription Polymerase Chain Reaction, Western Blot